Abbott and Costello were a 20th century American comedy duo who were the most popular comedy team in vaudeville, radio, film and stage during the 1940s and early 1950s. The duo consisted of Bud Abbott and Lou Costello.

A week before Benny Russell and his classmates visited the 1939 New York World's Fair in 1940, Abbott and Costello had performed at the fair's main pavilion, as had the Marx Brothers. (DS9 novelization: Far Beyond the Stars)

On June 1, 1944, Brigitte compared the temporally displaced Jake Sisko and Nog to Abbott and Costello as she regarded them as being comically mismatched due to the difference in their respective heights. Neither Jake nor Nog had ever heard of the duo. (DS9 novel: Trapped in Time)

Charles Tucker III was a fan of the Abbott and Costello film Abbott and Costello Meet Frankenstein. (ENT episode: "Horizon")

Miles O'Brien would joke with Keiko O'Brien that she was the Abbott to his Costello due to the differences between them, although Keiko did not understand the reference as Abbott and Costello references were considered to be archaic in the 24th century. (DS9 novel: The Siege)

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