Barbara could refer to:
- People
- Barb, a mercenary in the Silver Paladin group
- Barbara, a significant person to Captain Mike Laird of the USS Indomitable
- Barbara Asanjian, colony physician on Beta Trilochus
- Barbara Attias, an engineering technician aboard the USS Enterprise
- Barbara Bonnie, an ensign who boarded the Northwest Passage
- Barbara Brinson, a lieutenant aboard the USS Vancouver
- Barbara Covington, a Starfleet casualty of the Dominion War
- Barbara Einicrox, a 23rd century Federation President
- Barbara Ellison, a farmer on Sheridan's World
- Barbara Evans, a science officer aboard the USS Enterprise-D
- Barbara Gore, daughter of David Gore, a colonist on Sheridan's World
- Barbara Hollitt, a doctor sent to investigate the planet Velex
- Barbara Kesel, an assistant engineer aboard the USS Enterprise-D
- Barbara Leighton, founder of the Manitoba Journal of Interplanetary Psychology
- Barbara McCoy, a xenozoologist, daughter of Leonard McCoy
- Barbara O'Marla, an officer aboard the Plush Princess
- Barbara Robinson, a security officer aboard the USS Voyager
- Barbara Rossi, a flight controller aboard the USS Enterprise-E
- Barbara Smith, a yeoman aboard the USS Enterprise
- Barbara Winston-Smyth, a flight controller aboard the USS Enterprise-D
- Barbara Zannic, a geologist on Sheridan's World
- Androids
- Barbara series, a series of androids on planet Mudd
- Barbara 4
- Barbara 322
- Barbara series, a series of androids on planet Mudd
- Locations
- Santa Barbara, a city in California on Earth
- Santa Barbara Island, a small island not far from the city of Los Angeles
- Real-world topics
- Barbara Hambly, a TOS novelist
- Barbara March, TNG and DS9 actress who portrayed Lursa
- Barbara Paul, a TOS novelist
- Barbara Siegel, a TOS young adult novelist
- Barbara Strickland, a TNG young adult novelist
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