Gladius could refer to:
- gladius
- Gladius-class escort, a class of escort used by Starfleet in the early 25th century, derived from the Saber-class.
- Gladius-class frigate
- Gladius-class fighter, a class of fighter used by the Romulan Star Empire in the 2250s of the alternate reality created by Nero's temporal incursion
- Gladius (Mirak)
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