By the 2260s the standard Starfleet hypospray was designed to accept a swappable vial of medicine. Field hyposprays were pre-loaded with an inert saline solution that could carry any of five selected concentrated emergency medication. (TNG reference: Star Trek: The Next Generation Technical Manual)
In the early 23rd century the standard Starfleet hypospray held one ampule which held ten doses of a single drug. By the 24th century this capacity was increased to five ampules each holding ten doses. (Decipher RPG module: Player's Guide)
In 2259 of the Kelvin timeline, Spock injected the Gorn commander with a hypospray full of the antidote to the Gorn virus, resulting in his death just as he, Kirk and T'Mar beamed away from the Gorn mothership. (TOS video game: Star Trek)
In 2270, Montgomery Scott rigged a hypospray to explode on impact and emit anesthesia gas. James T. Kirk threw it at a tribe of Xhosa who were trying to kill his otherwise unarmed landing party on Vehtora IV. Unfortunately the tribesmen appeared to be immune to the gas. (TOS - Star Trek Unlimited comic: "As Flies to Wanton Boys")
- Hyposprays have appeared in every Star Trek series thus far.