Nightingale could refer to:
- Florence Nightingale, a 19th century British social reformer and nurse
- Nightingale (bird), a variety of bird
- Kainji nightingale, a variety of nightingale
- Starships:
- USS Nightingale (NCC-192), a Daedalus-class scout
- USS Nightingale (medical supply ship), a medical supply ship
- USS Nightingale (NCC-60805), a Nebula-class starship
- USS Nightingale, a Wallenberg-class transport
- Medical Transport 136, also known as the Nightingale
- Nightingale (episode), a Voyager episode
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