Roanoke Colony was the first European settlement established in North America. It was inhabited by British colonists in the Outer Banks of North Carolina in 1585.
Several vessels were named after the colony, including the Galaxy X-class USS Roanoke, the runabout USS Roanoke and the 23rd century Roanoke colony transport ship.
In 1587, the Roanoke colony and all of its colonists disappeared without a trace. Colonists left behind only one clue to their fate: the word "Croatoan". (TNG novel: Grounded)
In 2267, the USS Enterprise's historian Specs equated their recent discovery of Wanderer, a lost Earth multi-generation colony ship, to having found the lost Roanoke Colony. (TOS novel: The Galactic Whirlpool)
In 2368, Lieutenant Commander Data compared the mysterious disappearance of Roanoke Colony to the USS Enterprise-D's investigation of the fate of a Federation scientific station and its staff on Phaedra. (TNG novel: Grounded)